Author

Yanfeng Liu

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#gene editing Oct 2026

Replicon engineering-driven modular synthetic biology toolkit for efficiently iterative genome editing in Fusarium venenatum.

Fusarium venenatum is the most widely used filamentous fungus for biomass-based microbial protein production mainly used as meat analogs. However, insufficient iterative genome editing and lack of synthetic biology tools for fine-tuning gene expression limited engineering of F. venenatum for improved traits. Here, repl...

S. Gu, Na Wang, Ya-Dong Tu et al. · Metabolic Engineering · 0 citations

Open access Aug 2026

De novo L-(+)-tartaric acid biosynthesis in multi-modular engineered yeasts

L-(+)-tartaric acid (L-TA) is a high-value chiral organic acid essential for food and pharmaceuticals. Despite its industrial importance, sustainable green production is constrained by the lack of a fully defined biosynthetic pathway. Here, we report the de novo biosynthesis of L-TA in Saccharomyces cerevisiae through...

Xuan Zhou, Jiaheng Hou, Zi-Kai Wang et al. · Nature Communications · 0 citations

Jul 2026

Plasmid- and Inducer-Free Biosynthesis of Difucosyllactose through Rational Fusion Enzyme Engineering and Systematic Pathway Optimization.

Difucosyllactose (DFL), a fucosylated human milk oligosaccharide, exhibits significant application potential in the food and pharmaceutical industries. However, the microbial production of DFL faces challenges such as the accumulation of the intermediate 2'-fucosyllactose (2'-FL) and the high costs associated with the...

Zhiqiang Liu, Binglin Li, Jie-Ying Deng et al. · ACS Synthetic Biology · 1 citation

Jul 2026

Computationally Guided Engineering of Multi-Enzyme Cascades Enables Efficient Trehalose Biosynthesis.

Trehalose is a nonreducing disaccharide widely used for its biomolecule-protective properties. However, multienzyme cascade production remains limited by low enzyme expression and suboptimal catalytic performance. To address this, thermostable maltooligosyltrehalose synthase (TreY) and trehalohydrolase (TreZ) from Arth...

Haidong Huang, Yang-Yang Li, Jin-Song Song et al. · Journal of Agricultural and... · 1 citation

Jul 2026

Precursor supply and translational machinery engineering of Saccharomyces cerevisiae for improving cellular protein content and biomass-based microbial protein bioproduction.

A multilevel engineering strategy to enhance yeast protein production by optimizing precursor supply, translation machinery, and diploid construction provides both improved protein content and key targets for breeding high-protein microbial strains.

Yang Liu, Cai-Ying Jin, Wentao Shen et al. · Bioresource Technology · 1 citation