A visual system for assessing genome-editing efficiency in cotton using GhF3H, a key gene involved in anthocyanin biosynthesis whose knockout results in distinct changes in tissue coloration, that enables rapid visual screening of editing events within 45 days, facilitating the comparison of CRISPR toolkits, validation...
Yan-Li Chen, Qi Ma, Ji-Lian Li et al.· Journal of Cotton Research· 0 citations
Abstract Upstream open reading frames (uORFs) are intrinsic regulatory elements that fine-tune gene expression at the translational level without altering genomic sequences or protein structures. Here, we targeted the conserved uORF that resides in the 5'-untranslated region (5'-UTR) of a GDP-galactose phosphorylase ge...
Glyphosate, one of the most widely used and effective herbicides worldwide, plays a critical role in weed management. However, its application can indirectly compromise crop yield and fiber quality while controlling weeds. Consequently, breeding crop varieties that simultaneously exhibit high herbicide tolerance, high...
Peng Wang, Meng-Meng Li, Juyun Zheng et al.· Frontiers in Plant Science· 0 citations
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