Transposon end recognition and pairing by I-F3 CRISPR-associated transposase
To develop gene therapy tools based on CRISPR-associated transposons (CASTs), it is essential to define how transposon ends are recognized and paired during transposition. Transposon 7 (Tn7)–like transposons typically contain asymmetric left- and right-end sequences that flank and define DNA cargo. However, how the tra...