Author

Traver Hart

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#gene editing Open access Sep 2026

Combinatorial CRISPR/Cas12a excises targeted loci in the mammalian genome at very low efficiency

Findings indicate that the Cas12a system does not yield locus excision at the efficiency required for sensitive negative selection assays, and showed no meaningful correlation between gRNA efficiency, orientation, GC content, or cut site geometry and the success of locus excision.

N. Anvar, Zsofia M. Szegletes, R. Steger et al. · bioRxiv · 0 citations

Jul 2026

Abstract B093: Combinatorial CRISPR screening reveals synthetic lethal and suppressor interactions in RTK signaling and glycosylation networks

The N-glycosylation module is demonstrated as a recurrent hub of context-dependent genetic interactions and combinatorial CRISPR screening as a scalable approach for identifying therapeutic targets and resistance mechanisms in oncogene-driven cancers.

Subin Kim, Chen-Chu Lin, Sabriyeh Alibai et al. · Clinical Cancer Research · 0 citations