CRISPR/Cas9-Mediated MSTN Knockout Alters Muscle Proteome and MSTN–ACVR2B Interaction in Buffalo Myoblasts
Myostatin (MSTN) is a potent negative regulator of skeletal muscle growth and differentiation. Buffalo myoblasts were isolated from the longissimus dorsi muscle and characterized by binucleate morphology and Pax7/myosin immunostaining. Myoblasts were nucleofected with MSTN guide RNA and Cas9, and three independently de...