Exploiting HSD17B11-dependent dialkynylcarbinols cytotoxicity for facile CRISPR/Cas9-based gene inactivation
It is demonstrated that co-inactivation of HSD17B11 followed by PAC selection can be used to rapidly identify efficient guide RNAs targeting a gene of interest and to readily isolate clones inactivated for one or multiple genes.
Bastien Dumais, Madeleine Bossaert, Patrick Seigneur et al.
· bioRxiv · 0 citations