Sheng-Ming Yang

CRISPR/Cas9-Mediated Mutagenesis of Two Alleles Encoding a Glutathione S-Transferase Confirms Their Identity as Fhb7 Conferring Fusarium Head Blight Resistance in Wheat.

GST was confirmed as the causal gene for Fhb7-mediated FHB resistance and the power of wheat × maize hybridization coupled with genome editing in validating gene function in wheat was demonstrated.

Genome-wide association mapping of tan spot resistance in a modified NAM population derived from synthetic hexaploid wheat

Genetic dissection of seedling resistance to septoria nodorum blotch resistance in a nested association mapping panel derived from synthetic hexaploid wheat lines and bread wheat.

Septoria nodorum blotch (SNB), caused by the necrotrophic fungal pathogen Parastagonospora nodorum, is a major foliar disease of wheat (Triticum aestivum) that significantly reduces grain yield and quality. In this study, we evaluated a modified nested association mapping (NAM) population for SNB resistance. This popul...

The hijacking of a wheat Exo70-like protein by a fungal pathogen triggers disease

It is demonstrated that elimination of the tan spot susceptibility gene Tsc2 through conventional breeding using a diagnostic marker or disruption of Tsc2 by gene editing can be used to improve tan spot resistance in wheat.

Targeted Gene Editing in Wheat During Haploid Production via Wide Hybridization with Transgenic Maize Expressing Cas9 and Guide RNA.

A stepwise protocol for targeted gene editing in wheat via wide hybridization with transgenic maize expressing Cas9 and gRNA is described, which provides a one-step approach for generating DH lines with the target gene edited from any wheat genotypes of interest.