2026
Targeted Gene Editing in Wheat During Haploid Production via Wide Hybridization with Transgenic Maize Expressing Cas9 and Guide RNA.
A stepwise protocol for targeted gene editing in wheat via wide hybridization with transgenic maize expressing Cas9 and gRNA is described, which provides a one-step approach for generating DH lines with the target gene edited from any wheat genotypes of interest.
Shaobin Zhong, Y. Leng, Shengming Yang
· Methods in molecular biology · 0 citations