Author

S. De Rouck

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Open access Sep 2026

Multiparental RNA-seq driven eQTL screening identifies loci underlying host plant fitness in a generalist herbivore

The two-spotted spider mite (Tetranychus urticae) is an extremely polyphagous pest, yet the genetic basis of this adaptive potential remains to be fully elucidated. Since expression quantitative trait loci (eQTLs) provide the genetic basis of numerous phenotypes, we aimed to identify trans-eQTL hotspots underlying T. u...

Femke De Graeve, Berdien De Beer, S. De Rouck et al. · bioRxiv · 0 citations

Open access Sep 2026

Interfering with DNA repair pathways to enhance CRISPR-Cas9-mediated homology-directed repair in a chelicerate genetic model

This work provides the first functional analysis of double-strand break repair in a chelicerate of T. urticae, and demonstrates that Polθ-deficient strains enhance incorporation of repair templates, even when mutations are distant from the cut site.

S. De Rouck, W. Dermauw, T. van Leeuwen · iScience · 0 citations

#gene editing Open access Aug 2026

Gene knockout of P450s CYP392A16 and CYP392D2 reveals unexpected in vivo effects and transcriptional rewiring in the extreme generalist Tetranychus urticae.

It is demonstrated that even resistance-associated detoxification genes that are considered functionally validated in vitro, can have strong context-dependent phenotypic effects in vivo, underscoring the complexity of resistance gene networks and highlighting the need to interpret CRISPR/Cas9 knockout phenotypes within...

S. Naessens, S. De Rouck, Marilou Vandenhole et al. · Insect Biochemistry and Mole... · 0 citations