Optimized gGBE enables robust G-to-Y conversion in mammalian cells and zebrafish embryos.
This study engineered a series of glycosidase guanine base editor (gGBE) constructs by inlaying the N-methylpurine DNA glycosylase (MPG) domain into Cas9 protein and generated zebrafish models carrying mutations homologous to human amyotrophic lateral sclerosis (ALS)-associated variants.
Min Li, Yao-Ge Jiao, R. Tao et al.
· Zoological Research · 1 citation