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This protocol enables the generation of stable gene-edited alfalfa plants from shoot tips and significantly reduces the time required, providing a robust and practical platform for optimizing stem regeneration-based genetic modification systems.
Current progress is best interpreted as the engineering of interacting delivery and regeneration constraints, not as a universal transition to genotype-independent transformation, which is best interpreted as the engineering of interacting delivery and regeneration constraints.
Understanding of the characteristics of the CkPIP family is expanded and valuable molecular insights for drought-resistance research and forage improvement are provided.
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