Open access
Jul 2026
The impact of evolutionary dynamics and expression plasmid stability on long-term heterologous protein production by yeast
The results support a model in which prolonged expression of a burdensome secreted recombinant protein can be associated with early non-genetic heterogeneity in intracellular mCherry fluorescence, growth advantages of low-fluorescence cells and evolution of plasmid variants that reduce expression.
Ming-Zhi Xu, Andrei Parker, K. Winzer et al.
· Microbial Cell Factories · 0 citations