Precise genomic integration of large DNA fragments by donor-directed annealing using prime editing.
A programmable gene replacement tool, named prime assembly (PA), which adapts prime editors to produce one or two pairs of 3'-flaps on both the genome and donor DNA, allowing megabase-scale genomic excision and/or kilobase-scale donor insertion at the gene of interest.
Ho-Jun Jung, Bada Jeong, Yong-Woo Kim et al.
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