Oct 2026
Systematic identification of permissive insertion sites for foreign gene expression in a NADC30-like PRRSV infectious clone and development of dual-fluorescent recombinant viruses.
This study identifies the NSP1β/NSP2 and ORF1b/ORF2 sites as suitable and genetically stable insertion points for foreign genes within the NADC30-like PRRSV genome, providing an experimental basis for optimizing PRRSV-based foreign gene expression vectors and developing multi-gene expression platforms.
Ya-Wei Sun, Meng-Xin Zhang, Jia-Hui Ma et al.
· Veterinary Microbiology · 0 citations