Lisandro Pacheco Lugo

CRISPR/Cas9-based functional genomics in Trypanosoma cruzi: a systematic review of tools, applications, and biological findings

Trypanosoma cruzi, the causative agent of Chagas disease, was for decades one of the most genetically intractable organisms among trypanosomatids, owing to a highly repetitive, multigene-family-rich genome, frequent aneuploidy, absence of a functional non-homologous end-joining repair pathway, and the low efficiency of...