Engineering GFPuv Reporters for Visual Screening across Filamentous Fungi to Enable CRISPR Genome Editing in Aspergillus pseudoterreus
GFPuv is a UV-excitable fluorescent reporter that enables rapid monitoring of gene expression, particularly in microbial systems. However, its application in filamentous fungi remains limited. Here, we evaluated GFPuv variants and identified one that performs robustly across Aspergillus species and the yeast Yarrowia...