Author

Kedric L. Milholland

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Open access Mar 2026

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens

A new series of template vectors suitable for employing AID2 technology in prototrophic C. albicans strains, such as clinical isolates and the reference strain SC5314 are reported, and it is demonstrated that the AID2 system also works in Candida auris, albeit less effectively under some conditions.

E. Danzeisen, Michelle V. Lihon, Kedric L. Milholland et al. · bioRxiv · 0 citations

#gene editing Open access Aug 2026

Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens

ABSTRACT The auxin-inducible degron (AID) technology is a convenient and powerful tool for protein functional characterization in a broad array of eukaryotic species. We recently demonstrated that the original AID and improved AID2 systems are very effective at rapid protein depletion in Candida albicans, and described...

E. Danzeisen, Michelle V. Lihon, Kedric L. Milholland et al. · Msphere · 0 citations