Optimizing CRISPR–Cas9-mediated TCR knockout in primary mouse CD8+, CD4+, and regulatory T cells
CRISPR–Cas9 genome editing has transformed biomedical research by enabling precise and efficient genetic modification. Among its applications, gene knockout using ribonucleoprotein (RNP) complexes provides a non-viral strategy for introducing loss-of-function mutations. Here, we present an optimized CRISPR–Cas9 RNP-bas...
Andrea Šarac, Klemen Kunej, Kristina Manzoni et al.
· Scientific Reports · 0 citations