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Conventional and transgenic approaches to crop improvement are increasingly constrained by biosafety concerns, stringent regulatory frameworks, and limited public acceptance, necessitating new precision breeding methods. DNA-free genome editing using the CRISPR/Cas9 approach has emerged as a transformative approach t...
This review synthesizes current progress in citrus improvement by integrating tissue culture, genetic transformation, genome editing, and metabolomics approaches, and provides a comprehensive framework for modern citrus improvement.
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