U-box-type E3 ubiquitin ligases (PUBs) are critical for signaling homeostasis and abiotic stress responses via substrate ubiquitination, yet their upstream transcriptional regulation remains largely unexplored, especially in alfalfa drought adaptation. Through GWAS of 171 alfalfa accessions, we identified a novel U-box...
ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...
This study generated 528 RNA‐seq libraries from 176 alfalfa accessions under well‐watered and salt stress conditions and functionally validated MsRD26, a NAC transcription factor, as a major candidate positive regulator of salt tolerance.
Lin Chen, Yuqi Zhang, Xin-Yue Ma et al.· Advancement of science· 1 citation
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