Molecular dynamics simulation and QM/MM study of IscB-ωRNA complex
The CRISPR-Cas9 system's gene-editing fidelity relies on the HNH nuclease domain's ability to hydrolyze DNA via metal-ion catalysis. While IscB-ωRNA variants offer advantages for viral delivery due to their small size, their editing efficiency is much lower than Cas9. The understanding of how HNH-domain dynamics and me...
Ganggang Bai, Buyong Ma, Jingjing Li et al.
· AIP Publishing · 0 citations