Genome edited sheep generated by zygote electroporation of prime editor ribonucleoproteins
Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3 R...
Zhi-Xuan Yu, Jun-Jin Li, Meng-Hao Liu et al.
· Frontiers in Genome Editing · 0 citations