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J. Zhai

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Open access Jul 2025

A conserved small RNA-generating gene cluster undergoes sequence diversification and contributes to plant immunity

Small RNA-mediated gene silencing contributes to plant immunity. The secondary small interfering RNA (siRNA) pathway promotes defense by silencing target genes in invading fungal and oomycete pathogens. Many secondary siRNAs derive from transcripts potentially encoding pentatricopeptide repeat (PPR) proteins. Here, we...

Li Feng, Ying-Nan Hou, AmirAli Toghani et al. · 3 citations

Construction of Specific Parallel Amplification of RNA Ends (SPARE) libraries for the systematic identification of plant microRNA processing intermediates

This method, called SPARE (Specific Parallel Amplific Parallel Amplification of 5 0 RNA Ends) allows the identification of processing intermediates for most of the Arabidopsis miRNAs and enables the determination of the DCL1 processing direction and the cleavage sites introduced by miRNA processing machinery in the precurs...

Arnaldo L. Schapire, N. Bologna, B. Moro et al. · 1 citation
Open access Aug 2026

A conserved small RNA-generating gene cluster undergoes sequence diversification and contributes to plant immunity

It is reported that siRNA production is an ancient function of a conserved clade of a conserved clade of PPR genes that undergo extensive within-species diversification and accumulation of high-impact variations including pseudogenization, leading to the accumulation of a diverse siRNA pool.

Li Feng, Yingnan Hou, AmirAli Toghani et al. · 1 citation
Open access Jul 2026

Cell-type specific early perception of nine phytohormones revealed by single-nucleus transcriptomics in Arabidopsis

Cell-type-specific responses of Arabidopsis seedlings to nine phytohormones are described, characterize transcriptional crosstalk among them, and identify a guard-cell MYB60 module linked to stomatal regulation.

Zhijian Liu, Zhuowen Li, Yuzhuo Wang et al. · 0 citations
Review Jun 2026

RNA regulation in plants.

The roles and molecular mechanisms of diverse RNA types, the roles of RNA structures and modifications in regulatory processes, and the translational application of RNA-based strategies for improving agronomic traits are discussed.

Yijun Qi, Yue-Qin Chen, Hongwei Guo et al. · 0 citations

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