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A broad-acting mechanism that allows Phikmvvirus phages to evade nuclease targeting is described and phage escape through faithful repair is described and a potentially interesting gene for phage therapy is identified.
Summary Bacteriophage genomes are densely packed with coding sequences and frequently encode genes of unknown function. Unbiased phage functional genomics approaches are therefore needed, particularly for large lytic phages. Here, we harness the mariner transposase to develop phage transposon mutagenesis and sequencing...
Jumbo bacteriophages in the ϕKZ-like family have shown great potential as a therapeutic to treat infections from the human pathogen Pseudomonas aeruginosa, known for its high level of antibiotic resistance. ϕKZ-like phages form a proteinaceous “phage nucleus” upon infection that compartmentalizes and protects pha...
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