Genetic background determines allele-specific Pfkelch13-mediated artemisinin tolerance and persistence in Plasmodium falciparum
Artemisinin partial resistance (ART R ) in Plasmodium falciparum is mediated by Pfkelch13 mutations but has not emerged in West Africa. Using gene editing, we introduced four Pfkelch13 mutations into a recent Senegalese isolate, evaluating the receptivity of this genetic background to ART R using the ring-stage survival assay (RSA), a proxy for clinical resistance. One allele conferred the RSA phenotype but was rapidly outcompeted, whereas three conferred stable resistance, revealing allele-specific permissiveness
Imran Ullah, Morgan C. Martin, Daouda Ndiaye et al.
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