G. P. Dixit

Design and validation of CRISPR/Cas9 sgRNAs and vector construction for targeting the LsBOS gene in grasspea (Lathyrus sativus L.)

Grasspea is an important grain legume crop due to its hardiness and high protein content. However, the presence of a neurotoxic compound β -ODAP, which is associated with lathyrism, limits its widespread cultivation and consumption. In this study, the LsBOS gene, which encodes β -ODAP synthase, was PCR-amplif...