In vitro validation of a CRISPR/Cas9 guide RNA targeting COL1A1 gene by cleavage assay.
Functional validation of the gRNAs designed to target exon 2 of the COL1A1 gene is confirmed through in vitro cleavage activity and support its candidate selection for subsequent cellular studies targeting COL1A1, representing an initial step toward future knockout-style cellular models of Osteogenesis Imperfecta.
D. M. Melo, Sabrina Rodrigues Sarquis, Paolla Manzoli Luz et al.
· Methods · 0 citations