Repurposing Cas12a2-mediated abortive infection for efficient and low-toxicity microbial genome editing
CRISPR-Cas systems have revolutionized microbial genome engineering, yet counter-selection strategies typically rely on targeted DNA cleavage, where the severe cytotoxicity of double-strand breaks restricts transformation efficiency and clonal recovery. Here, we present an innovative strategy, SuCas12a2-Universal Recom...
Hao-Da Wang, Lan Hu, Jing Han et al.
· Nature Communications · 0 citations