Evaluating high-fidelity CRISPR-Cas nucleases in nucleosomal contexts using a quantitative framework
It is demonstrated that local nucleosome sequence and structure profoundly influence Cas nuclease accessibility and specificity, with HIFIv1 emerging as the top-performing nuclease for nucleosomal targets, while evoSpCas9 excelled in exposed contexts.
Christopher Handelmann, Erin Skeens, George P. Lisi et al.
· Frontiers in Genome Editing · 0 citations