An optimized pipeline for cell-based validation of gRNAs designs to inactivate coding and non-coding RNAs using CRISPR-Cas9
Genome-editing technologies based on bacterial Cas9 proteins have become a gold standard for introducing precise genetic modifications across multiple species and experimental models. These tools require the design and validation of guide RNAs (gRNAs) that direct the Cas9 protein to the actual genome location to be edi...
Florence Jaouen, Catherine Lépolard, Thomas Bescond et al.
· Scientific Reports · 0 citations