#gene editing
Aug 2026
Design of base editing systems with tunable editing hotspots within a defined window through sgRNA engineering.
Base editors (BEs) enable efficient A-to-G or C-to-T conversions without double-stranded DNA cleavage, but their editing windows remain difficult to tune, limiting genome engineering flexibility. Here, we engineered CRISPR/Cas12b sgRNA by introducing MS2 hairpins to recruit an MS2-N55K-cytidine deaminase-UGI complex, e...
Wen-Liang Hao, Lai-Chuang Han, Yao-Kang Wu et al.
· Microbiology Research · 0 citations