CRISPR/LbCas12a-Mediated Multiplex and Transgene-Free Genome Editing in Grapevine.
Agrobacterium ‐mediated transformation followed by regeneration without antibiotics‐dependent selection offers a a straightforward and effective approach for generating transgene‐free edited plants in grapevine, and the use of CRISPR/LbCas12a enables the simultaneous editing of multiple genes, thereby facilitating...