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Author

Ai-Qun Yu

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Aug 2026

A Dual-Dimensional CRISPR Toolkit Enables One-Step High-Efficiency Multiplex Genome Editing in Komagataella phaffii.

A CRISPR toolkit - Efficient Multi-Gene Editing System 3.0 (EMGES 3.0) - which enabled one-step large-fragment integration coupled with multiplex gene knockout coupled with multiplex gene knockout is established and provides a robust technical foundation for complex compounds biosynthesis and high-yield industrial stra...

Zhixian Yu, Kaidi Chen, Gulikezi Maimaitirexiati et al. · 0 citations
#gene editing Open access Sep 2026

Loss of Ku70 promotes mononucleate conidiation and homologous recombination in Phanerochaete chrysosporium

ABSTRACT Lignin is a major constituent of lignocellulose and the most abundant aromatic biopolymer on earth. It provides plants with rigidity and protection, but its recalcitrant nature also presents a significant barrier to lignocellulose valorization. The white-rot fungus Phanerochaete chrysosporium is among nature’s...

Hao Wu, Jiao-Jiao Zhang, Yi Zhao et al. · 0 citations

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