Experimental dataset for: The Role of miR-214-3p in Hair Follicle Neural Crest Stem Cell-derived Exosomes in the Repair of Peripheral Nerve Injury

Abstract

This dataset contains all raw and processed data supporting the findings of the original research article "The Role of miR-214-3p in Hair Follicle Neural Crest Stem Cell-derived Exosomes in the Repair of Peripheral Nerve Injury". The study aimed to investigate the role and mechanism of hair follicle neural crest stem cell-derived exosomes (hfNCSCs-Exos) and their carried miR-214-3p in promoting neurite outgrowth and peripheral nerve regeneration. The dataset comprises the following: 1. Cell and Exosome Characterization Data: Immunocytochemical staining images of primary hair follicle neural crest stem cells (hfNCSCs). Western Blot raw images and quantification data for exosome markers (e.g., CD63, CD9) of hfNCSCs-Exos. 2. In Vitro Experiment Data: Immunofluorescence staining images (for markers like GAP43 and NF200) of acrylamide-injured rat dorsal root ganglion (DRG) neurons treated with different groups (Control, hfNCSCs-Exos, miR-214-3p HA/LA hfNCSCs-Exos). Includes raw image files for quantification and analyzed morphometric data (e.g., neurite length, branch count). 3. In Vivo Experiment Data: Histological Data: Immunohistochemical/immunofluorescence staining images (e.g., for NF200) of longitudinal sections from the regenerated sciatic nerve in a rat defect model across different treatment groups. Functional Recovery Data: Histological section images (H&E staining) of gastrocnemius. Raw records and quantified scoring sheets for motor function assessments (e.g., Sciatic Functional Index). Methodology & Processing: Data were generated following the experimental procedures detailed in the "Materials and Methods" section of the associated paper, including cell isolation, exosome extraction/characterization, establishment of cell and animal injury models, immunostaining, Western Blot, image acquisition, morphometric analysis, and behavioral tests. Raw images were analyzed using Fiji software. Link to Publication: This dataset directly supports the research presented in the manuscript titled "The Role of miR-214-3p in Hair Follicle Neural Crest Stem Cell-derived Exosomes in the Repair of Peripheral Nerve Injury ". The DOI for the article will be added upon acceptance.