Evaluation of Gelatin/Xanthan Gum Based Biomaterial Enhanced with Barbaloin for Wound Healing Application: An In silico and In vitro Study

Abstract

In this study, a gelatin- and xanthan-gum-based biomaterial enhanced with barbaloin was developed and evaluated for its potential application in wound healing. The scaffold was designed to leverage the biocompatibility of gelatin, structural stability provided by xanthan gum, and therapeutic properties of barbaloin. Physicochemical characterization was conducted using FTIR, XRD, and SEM. XRD patterns indicated an amorphous structure, favorable for enhanced swelling and controlled drug release, whereas FTIR confirmed effective molecular interactions among the components. SEM imaging revealed a porous and interconnected architecture that is essential for cell migration and nutrient exchange. Swelling and porosity assessments demonstrated the ability of the scaffold to efficiently absorb wound exudates and maintain a moist environment conducive to tissue regeneration. Biocompatibility, evaluated via the MTT assay, showed high fibroblast viability, confirming the non-toxic nature of the material. Furthermore, molecular docking studies revealed strong binding affinities between barbaloin and wound healing-related proteins, suggesting its role in modulating inflammation and promoting tissue repair. Overall, the Gelatin/Xanthan Gum/Barbaloin scaffold exhibited excellent physicochemical characteristics, cytocompatibility, and therapeutic potential, making it a promising candidate for advanced wound dressing applications.