Clinical Isolates Identification of Burkholderia Species and Evaluate Their Antibiogram at a Tertiary Care Hospital

Abstract

Background: The genus Burkholderia, which was previously believed to be a soil saprophyte, is now gaining attention as a human disease. Pathogenic species include B. pseudomallei, B. cepacia, B. mallei, B. gladioli, and B. thailandensis. Ongoing studies on Bukholderia have led to the isolation of more recent subspecies from human samples. The purpose of this study is to assess the antibiogram of the clinical isolates and determine the prevalence of Burkholderia spp. Methods: Demographic data and a variety of patient clinical samples were analyzed. All specimens were processed using recognized microbiological techniques. Results: Of the 8230 culture-positive samples, 1902 (23.11%) included Non-Fermenting Gram Negative Bacilli (NFGNB). It was discovered that 60 (3.2%) of these NFGNB were Burkholderia spp. Furthermore, it was found that 24 (40%) and 36 (60%) were B. pseudomallei and B. cepacia complex, respectively. Of the patients, 76.7% were over 40, and 80% were men. Diabetes mellitus was the primary risk factor (60%), and fever was the most common manifestation (53.3%). Imipenam had the lowest sensitivity, while Minocycline and Cotrimoxazole had the highest, according to antibiotic sensitivity testing. Conclusion: This study provides baseline data on the present Burkholderia infection situation at our hospital. An ongoing investigation will be useful in identifying several cases because this organism is extremely underreported.