Enhanced biocontrol of postharvest blue mold in apple by phytopathogen targeted Bacillus subtilis 507.

Systematic Investigation of the Biocontrol Potential of Bacillus velezensis B05 against Soybean Root Rot Pathogen Fusarium spp.

Results indicate that B. velezensis B05 has potential as a multifunctional biocontrol agent for sustainable management of soybean root rot.

Biocontrol efficacy of Bacillus albus SSR3 for controlling postharvest fungal pathogens and mycotoxin contamination.

Sweetpotato black rot, caused by Ceratocystis fimbriata, is a major postharvest disease that leads to substantial storage losses worldwide. In this study, a salt-tolerant rhizobacterial strain, Bacillus albus SSR3, was isolated from the rhizosphere of sweetpotato grown in saline-alkali soil, with broad-spectrum antagonistic activity against postharvest fungal pathogens. LC-MS/MS analysis revealed diverse bioactive metabolites associated with its antifungal activity. Integrated transcriptomic and metabolomic analyses showed that SSR3 bioactive metabolites extensively reprogrammed fungal metabolism, particularly pathways involved in carbohydrate and amino acid metabolism, antioxidant defense, and energy production. These alterations were accompanied by disruption of cell wall and membrane integrity, excessive reactive oxygen species accumulation, and mitochondrial dysfunction, ultimately inhibiting fungal growth. Here, we also found that SSR3 bioactive metabolites effectively inhibited aflatoxin B1 production by Aspergillus flavus and deoxynivalenol accumulation in Fusarium graminearum. In vivo assays further demonstrated that SSR3 bioactive metabolites significantly reduced sweetpotato black rot severity and effectively limited fungal colonization and mycotoxin contamination in stored agricultural commodities. Collectively, our findings demonstrate that B. albus SSR3 suppresses postharvest fungal pathogens through coordinated metabolic reprogramming, oxidative stress induction, and cellular integrity disruption, highlighting its potential as a sustainable biocontrol agent for postharvest disease management.

Biocontrol Efficacy of a Seed-Derived Streptomyces pseudogriseolus YTU-S40 Against White Rot in Postharvest Grapes

Grape white rot, a fungal disease caused by Coniella diplodiella, leads to the decay and abscission of leaves and fruits. This disease is recognized as one of the major fungal diseases affecting the grape industry, significantly impacting the quality and yield of grapes. In this study, a seed-derived Streptomyces strain with high biocontrol activity, designated YTU-S40, was isolated from the seeds of Cnidium monnieri (L.). Molecular phylogenetic identification revealed that this isolate belongs to Streptomyces pseudogriseolus. In vitro plate confrontation experiments demonstrated that YTU-S40 exhibited a robust inhibition rate of 76.2% against C. diplodiella. Furthermore, this strain exhibited relatively broad-spectrum antifungal activity, and genomic analysis predicted that its genome harbors 21 biosynthetic gene clusters responsible for secondary metabolite production. In vivo postharvest grape biocontrol assays verified that YTU-S40 significantly reduced the incidence of grape white rot from 94.4% to 1.9%. Untargeted metabolomic profiling suggested that YTU-S40 may produce bioactive antifungal compounds such as antimycins. Collectively, these findings suggest that YTU-S40 holds considerable promise for development as a biocontrol agent.

Laboratory evaluation of enhanced biocontrol efficacy of Bacillus atrophaeus SF9 via fermentation optimization against Bauhinia blakeana leaf spot disease caused by Colletotrichum endophyticum

Biocontrol potential of endophytic bacteria against a collection of Leptosphaeria maculans isolates causing blackleg in oilseed rape

Genome based characterization of an endophytic Bacillus subtilis BS01 associated with suppression of Fusarium wilt in Cavendish banana caused by Fusarium oxysporum f. sp. cubense Tropical Race 4

Genome mining revealed eight biosynthetic gene clusters associated with antimicrobial secondary metabolites, supporting the genomic characterization of strain BS01 and identifying its biosynthetic potential and identifying its biosynthetic potential.