This study systematically characterized the BBX family in T. grandis for the first time and identified key drought-responsive genes, providing valuable resources for drought-tolerant molecular breeding of this economically important gymnosperm.
Abstract
B-BOX (BBX) transcription factors regulate plant growth and drought tolerance, whereas comprehensive characterization of BBX gene families in gymnosperms remains scarce. We identified 13 TgBBXs in Torreya grandis at the genome-wide level, and phylogenetic analysis grouped them into five subgroups consistent with the conserved classification of plant BBX members. Promoter analysis revealed abundant light hormone and stress related cis-elements, and the TgBBXs displayed distinct expression patterns across various tissues and reproductive developmental stages. PEG-triggered drought decreased leaf chlorophyll, accompanied by increased root H2O2, APX and POD levels. Transcriptome data showed obvious tissue-specific expression and organ-dependent drought responses; most of TgBBX5–TgBBX12 were continuously upregulated in leaves under PEG treatment, whereas several members declined in roots. WGCNA further indicated that the MEturquoise module, which contained most TgBBXs, was closely associated with chlorophyll accumulation and antioxidant responses. Among these genes, TgBBX2, TgBBX7, and TgBBX13 with module membership over 0.9 are putative hub genes, and their associated genes enrich in photosynthesis and antioxidant pathways. This study systematically characterized the BBX family in T. grandis for the first time and identified key drought-responsive genes, providing valuable resources for drought-tolerant molecular breeding of this economically important gymnosperm.
It is demonstrated that heterologous expression of TksPLATZ1, TksPLATZ2 and TksPLATZ7 localize to the cell nucleus and act as transcriptional activators and repressors, respectively, which enhances the tolerance of Arabidopsis to salt and osmotic stress.
Jinxian Chen, Wenhao Wu, Ming-Hua Luo et al.· Phytochemistry· 0 citations
The Oxidative Stress 3 (OXS3) gene family encodes plant-specific proteins that play crucial roles in abiotic stress tolerance and chromatin remodeling. However, genome-wide identification and characterization of the OXS3 gene family in soybean have not been systematically conducted. Here, we identified 19 GmOXS3 genes from the soybean genome. Phylogenetic analysis assigned these genes to three subfamilies (I–III). Members within the same subfamily exhibited conserved motif compositions. The promoter regions of GmOXS3 genes contained various cis-acting regulatory elements associated with stress and phytohormone responses. Analysis of transcriptome data revealed that GmOXS3 genes exhibited different expression patterns in various organs. RT-qPCR further confirmed their differential expression under salt and alkaline stresses, with the most pronounced up-regulation observed for GmOXS3-1 and GmOXS3-14 under alkaline stress. Among them, GmOXS3-1 was further characterized, and it negatively regulates alkaline tolerance in soybean hairy roots. These results provide a foundation for elucidating GmOXS3-1-mediated alkaline stress signaling and highlight its potential as a breeding target for improving alkaline tolerance.
Xi Chen, Nai-Ze Mu, Ling-Shan Ren et al.· Agronomy· 0 citations
FaNAC6 was selected for its strong induction in leaves and roots in response to drought and salinity, as well as under oxidative stress and ABA, and was associated with the upregulation of genes involved in photosystems, electron transport and carbon fixation.
Facundo Spadoni-Revol, M. D. Moreno-Recio, Sara Aguado-Delgado et al.· International Journal of Mol...· 0 citations
Plant GH5 family genes function in both cell wall biosynthesis and stress responses. However, comprehensive studies on GH5 genes in the soybean remain limited. Here, we identified 28 GmGH5 genes from the soybean genome. Phylogenetic analysis assigned these genes to three subfamilies (I–III), with no representatives in subfamily IV. The GmGH5 family harbors 15 conserved motifs, which are largely similar within subfamilies but differ across subfamilies. Additionally, exon–intron structures (2–7 introns) exhibit clade-specific patterns, with members within the same clade sharing similar intron numbers and lengths, whereas distinct clades show some variation. The promoter regions of GmGH5 genes contained various cis-acting regulatory elements associated with stress responses and developmental processes. Transcriptome-based expression profiling revealed distinct tissue-specific expression patterns of GmGH5 genes. RT-qPCR further confirmed their differential expression under salt, alkaline, cold, and drought stresses, especially a significant increase in GmGH5-22 expression under salt stress (approximately 22-fold at 6 h, **** p < 0.0001). Furthermore, GmGH5-22 was highly expressed in roots, and transient expression in tobacco leaves showed its peripheral localization, which aligns with its predicted extracellular localization, suggesting that GmGH5-22 is highly likely localized to the cell wall. Overexpression of GmGH5-22 in soybean hairy roots significantly improved tolerance to salt stress. These findings establish a foundation for functional characterization of GmGH5 genes and provide viable targets for molecular breeding to enhance salt tolerance in soybeans.
Xi Chen, Ling-Shan Ren, Nai-Ze Mu et al.· Plants· 0 citations
This study elucidates the evolutionary conservation and functional diversity of the eggplant GATA family, providing valuable candidate genes for future functional research and stress-tolerant molecular breeding in eggplant.
Yang Huang, Li Jia, Kunyu Ma et al.· Horticulturae· 1 citation
The DUF789 protein family comprises conserved domain proteins in plants, most of which remain functionally uncharacterized despite their involvement in growth, development, and stress responses. Moreover, systematic identification of the DUF789 family in tomato (Solanum lycopersicum L.) has not been reported. In this study, 11 SlDUF789 members were identified from the tomato genome, unevenly distributed across seven chromosomes, with members within the same subgroup sharing similar exon–intron structures and conserved motifs. Promoter analysis revealed the enrichment of cis-acting elements associated with light, hormone, and stress responses. Quantitative real-time PCR (qRT-PCR) analysis revealed diverse expression patterns across tissues: most SlDUF789 members exhibited relatively high expression levels in reproductive tissues, whereas SlDUF789-7 was broadly expressed across all tissues, with elevated expression in fully open flowers and red-ripe fruits. Under stress and hormone treatments, SlDUF789-7 exhibited the highest induction under ABA and salt stress; SlDUF789-8 under MeJA and PEG; and SlDUF789-11 under SA and low-light. Notably, SlDUF789-7 responded strongly across all treatments, whereas SlDUF789-11 showed sustained upregulation under low-light stress. This study provides the first systematic characterization of the SlDUF789 gene family in tomato and establishes a foundation for future research on their biological functions and potential roles in stress responses.
Wen-Bin Zou, Xin-Fang Chen, Zhi-Peng Wang et al.· International Journal of Mol...· 0 citations
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