Porphyrin‑manganese trioxide-doped chitosan hydrogel with self‑oxygen supply enhancing photodynamic therapy effect for antibacterial and anti-inflammatory of infected wounds.
Aug 2026· International Journal of Biological Macromolecules· Vol 379, pp.
153901
· 0 citations· 26 references
Medicine
TL;DR
The antibacterial experiments results show that the combination of antibacterial and anti-inflammation mediated by TAPP/Mn3O4@CS-GA hydrogel can accelerate infected wound healing.
Abstract
To combat microbial infection and persistent inflammation of wounds, the increasing type and dosage of antibiotics in wound dressing results in the formation of drug-resistant bacteria, which seriously delays the healing of infected wounds. Thus, to develop alternative antibacterial wound dressings independent of antibiotics is imperative. In this work, we established a novel degradable hybrid nanohydrogel material (TAPP/Mn3O4@CS-GA) with antibacterial and anti-inflammation effects by loading 5,10,15,20-tetrakis (4-aminophenyl)-21H,23H-porphine (TAPP) with PDT function and Mn3O4 with SOD and CAT-like activities in chitosan hydrogel (CS-GA). The nanosystem can produce abundant ROS under laser irradiation, SOD and CAT-like activities of Mn3O4 generate enough O2 to provide substrate for PDT mediated by TAPP, increasing the concentration of singlet oxygen (1O2) and enhancing the PDT effect. After laser irradiation, SOD and CAT-like activities continue transfer superoxide anion free radical (O₂-·) and hydrogen peroxide (H2O2) to O2 to reduce inflammation caused by oxidative stress, relieve hypoxia and promote angiogenesis. The antibacterial experiments results show that the combination of antibacterial and anti-inflammation mediated by TAPP/Mn3O4@CS-GA hydrogel can accelerate infected wound healing.
This work demonstrates a safe and effective strategy for combating MDR infections through the combined action of photothermal therapy and nanozyme catalysis, offering promising potential for clinical wound management.
Weiwei Zhang, Lixiang Fan, Xuanjun Zhang et al.· ACS Applied Materials and In...· 0 citations
ABSTRACT Diabetic wounds are characterized by a complex pathological microenvironment, marked by persistent hypoxia, excessive reactive oxygen species (ROS), chronic inflammation, and bacterial infection. To address this challenge, we developed a multifunctional composite hydrogel (CFP/PC@MnO2) for diabetic wound healing. It is formed by covalent crosslinking of carboxymethyl chitosan (CMCS), 4‐formylphenylboronic acid (4FPBA), and polyvinyl alcohol (PVA) via Schiff base and boronate ester bonds, followed by incorporation of phycocyanin‐modified MnO2 nanoparticles (PC@MnO2 NPs). The CFP/PC@MnO2 hydrogel exhibits favorable mechanical properties, local injectability, self‐healing capability, tissue adhesion, and the ability to form a protective wound barrier. Meanwhile, the CFP/PC@MnO2 hydrogel degrades rapidly under ROS, releasing PC@MnO2 to scavenge ROS and generate O2 in situ to alleviate hypoxia; the PC@MnO2 NPs further exhibit strong NIR‐triggered photothermal antibacterial activity. In vitro, this hydrogel exhibits excellent biocompatibility, effectively scavenges ROS, alleviates oxidative stress, and suppresses inflammation. In a full‐thickness wound model in diabetic rats, the CFP/PC@MnO2 hydrogel combined with photothermal therapy effectively eradicates bacteria, promotes M2 macrophage polarization to modulate the immune microenvironment, and enhances angiogenesis and collagen deposition, thereby accelerating wound healing. Overall, this multifunctional hydrogel integrates ROS scavenging, self‐sustained oxygenation, immunomodulation, and photothermal antibacterial action, offering a synergistic, innovative strategy for diabetic wound treatment.
Zhi Xu, Xiaodong Luo, Jingxia Wu et al.· Advancement of science· 0 citations
Infected burn wounds are characterized by bacterial invasion, oxidative stress, and persistent inflammation, which severely impair tissue regeneration. Herein, we report a crosslinker-free, hydrogen-bonded cationic guar gum hydrogel (CBBM) co-loaded with berberine (BBR) and MnO2-coated black phosphorus nanosheets (BPNS@MnO2) for the microenvironment-adaptive treatment of infected burn wounds. The dynamic guar gum network endowed the hydrogel with injectability, self-healing ability, and conformal adaptability. BPNS@MnO2 exhibited pH-dependent enzyme-like activities, including OXD-like antibacterial activity under acidic conditions and SOD-/CAT-like ROS-scavenging activity under near-neutral conditions and endowed the hydrogel with NIR-triggered photothermal responsiveness. Moreover, NIR irradiation further enhanced the antibacterial efficacy and moderately enhanced BBR release from the hydrogel. In vitro, CBBM combined with NIR irradiation achieved potent antibacterial efficacy against S. aureus and E. coli under the tested conditions and reduced intracellular ROS levels. In vivo, the CBBM + NIR treatment accelerated infected burn wound healing, achieving a wound closure rate of 97.53 ± 2.01% by day 14, accompanied by reduced inflammation, enhanced collagen deposition, and increased expression of the angiogenesis-related markers VEGF and CD31. These results demonstrate that the CBBM hydrogel is a promising multifunctional guar gum-based dressing for infected burn wound healing by integrating local drug delivery, catalytic regulation, and photothermal activation.
Ziyi Zhao, Yanxiang Sang, Benyan Zheng et al.· International Journal of Bio...· 0 citations
A visible-light-responsive organic nanoplatform (iTPyPXs/SCM) that improves ROS utilization for antimicrobial therapy while limiting excessive intracellular ROS and provides a safer and more effective biomaterial strategy for photodynamic therapy in infected wound healing is developed.
Hongyu Lin, Qingyang Peng, Ying Lin et al.· Biomaterials· 0 citations
Skin wound infection is a key factor that hinders the healing process and repair outcome of wounds, especially when it involves drug-resistant bacteria, which is extremely difficult to eliminate. Therefore, developing wound dressings with the ability to kill drug-resistant bacteria holds great value. Herein, carboxymethyl chitosan (CMCS) and 2,4,6-trihydroxybenzaldehyde (THBA)@silver ion chelates were assembled into a triple dynamically cross-linked hydrogel (CTA) through imine bonds, hydrogen bonds, and polyphenol metal chelate bonds. The CTA0.15 hydrogel exhibits sensitive near-infrared response properties. When exposed to 808 nm near-infrared light with a power density of 0.3 W/cm2, the temperature can easily rise to between 45 °C and 50 °C, enabling photothermal sterilization. Combined with the strong antibacterial activity of silver ions and the photothermal effect of the hydrogel, it can quickly (15 min) and completely eliminate common pathogenic bacteria (E. coli and S. aureus) and drug-resistant bacteria (MRSA) in vitro. In addition, this hydrogel also possesses injectability, tissue adhesiveness, self-healing property, shape adaptability, antioxidant capability, catalase (CAT)-like activity, and biocompatibility. The in vivo experiments and transcriptome analysis demonstrated that the combination of CTA hydrogel and NIR treatment significantly accelerated the healing process of MRSA-infected wounds by eliminating antibiotic-resistant bacterial infections, regulating inflammatory responses, promoting collagen deposition and angiogenesis.
Chenghao Li, Pengyuan Liu, Pei Cheng et al.· International Journal of Bio...· 0 citations
The refractory healing of diabetic wounds represents a major clinical challenge, primarily attributed to a vicious cycle formed by persistent bacterial infection, excessive oxidative stress, and a dysregulated immune microenvironment. To simultaneously address multiple pathological barriers, an intelligent composite nanoplatform was designed and constructed in this study, integrating near-infrared (NIR) photothermal therapy, reactive oxygen species (ROS) scavenging, antimicrobial activity, and immunomodulation. A mesoporous polydopamine (MPDA) core was loaded with chlorogenic acid (CGA), a natural antioxidant, and further coated with a copper-zinc bimetallic organic framework (Cu/Zn-MOF) to fabricate Cu/Zn-MOF@CGA@MPDA NPs, exhibiting pH-responsive dissociation in the acidic infected wound microenvironment. Under 808 nm near-infrared irradiation, the fabricated NPs showed excellent photothermal performance, efficiently eliminating bacteria and biofilms in vitro. They also scavenged ROS to relieve oxidative damage and promoted macrophage polarization from the pro-inflammatory M1 to pro-healing M2 phenotype. In a diabetic rat model of infected full-thickness skin wounds, the nanoplatform achieved antibacterial and anti-inflammatory effects simultaneously. Such synergistic functions promoted collagen deposition and re-epithelialization, thereby accelerating diabetic wound healing. Histological and hematological tests verified its good biocompatibility and biosafety. This work developed a synergistic single-platform strategy for precise regulation of diabetic wound microenvironments, providing a promising therapeutic alternative for refractory diabetic wound treatment.