Aug 2026· Microbiology Research· Vol 313, pp.
128664
· 0 citations· 75 references
Medicine
TL;DR
It is suggested that FS treatment enhances the resistance of cucumber seedlings to Cd stress, and this effect may be associated with changes in the rhizosphere microbial community, activation of soil nutrients, and enhanced plant nutrient uptake.
Abstract
This study examines whether the mycorrhizal helper bacterium (MHB) Alcaligenes faecalis can enhance the cadmium resistance conferred by the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae in cucumbers. We evaluated plant growth, nutrient status, and rhizosphere microbiota across four treatments: non-inoculated control (CK), single inoculation of AMF (F), single inoculation of MHB (S), and co-inoculation of AMF and MHB (FS). Compared with the other three treatments, the FS treatment not only significantly increased the contents of N, P, and K in the plants, but also significantly increased the contents of soil nitrate N, available P, and available K. Moreover, the F, S and FS treatments have significantly decreased root Cd concentration and Cd content in the rhizosphere soil compared with the CK treatment. Different inoculation treatments have changed the relative abundance, composition and diversity of cucumber rhizosphere bacterial community. The F, S and FS treatments significantly increased Chao 1, Simpson index and number of OTUs compared with the CK treatment. The relative abundances of soil bacteria such as Ramlibacter, Mitsuaria, Flavisolibacter, Lysobacter, Sphingomonas and Massilia in the FS treatment were significantly higher than those in the other three treatments. Overall, our findings suggest that FS treatment enhances the resistance of cucumber seedlings to Cd stress, and this effect may be associated with changes in the rhizosphere microbial community, activation of soil nutrients, and enhanced plant nutrient uptake.
Arbuscular mycorrhizal fungi (AMF) and rhizobia establish mutualistic symbioses with plant roots. This study focused on their effects on alfalfa (Medicago sativa L.) growth, root morphology, nutritional quality, photosynthetic characteristics, and protein fractions; eight treatments were established: single inoculation with three AMF strains (Funneliformis mosseae, Claroideoglomus etunicatum, Glomus versiforme) or Sinorhizobium meliloti (Sm), dual co-inoculation of each AMF with Sm, and a non-inoculated control (CK). Results showed that all AMF successfully colonized alfalfa roots, with co-inoculation increasing both mycorrhizal colonization rate and nodule number. The F. mosseae × Sm treatment achieved the highest colonization (83.3%) and nodule count (76 per plant). Across two years, this treatment significantly increased aboveground biomass, plant height, and stem diameter (p < 0.05). C. etunicatum × Sm significantly reduced acid detergent fiber content, while dual inoculation markedly improved net photosynthetic rate and light-use efficiency. All inoculations increased rapidly (PB1) and intermediate-degradable protein (PB2) but decreased non-protein nitrogen (PA) and bound protein (PC). In conclusion, AMF and rhizobia exhibit significant synergistic effects. Co-inoculation (F. mosseae × Sm and C. etunicatum × Sm) enhances alfalfa productivity by optimizing root structure, improving photosynthesis, and regulating nitrogen metabolism.
Arbuscular mycorrhizal fungi (AMF) can be used as microbial fertilizers. Hydroponic cultivation offers significant advantages by reducing factors that cause damage during traditional soil-based cultivation. Furthermore, mycorrhiza helper bacteria (MHB) can improve mycorrhizal fungal colonization. The objective of this study was to examine the function of Funneliformis mosseae in promoting rice growth under soilless culture using a hydroponic cultivation system with two different levels of nutrient supply: A and B (high and low levels). Furthermore, Bacillus methylotrophicus was isolated from Funneliformis mosseae spores to examine its potential to promote rice growth in combination with AMF. The findings illustrated that three-month-old rice plants at the ripening stage inoculated with Funneliformis mosseae showed the highest increases in height, fresh weight of leaves and roots, and dry root weight under low levels of nutrient supply, with successful AMF root colonization of 78.67%. Additionally, co-cultivation of Funneliformis mosseae and Bacillus methylotrophicus significantly enhanced plant height, fresh weight of leaves and roots, and dry root weight under low levels of nutrient supply compared to uninoculated plants under high levels of nutrient supply. High levels of nutrient supply significantly decreased Funneliformis mosseae colonization of rice roots. These findings suggest that optimal levels of nutrient supply are necessary for rice growth with AMF colonization because high nutrient supply restricts plant growth and reduces AMF root colonization. However, Bacillus methylotrophicus did not demonstrate a strong relationship with AMF colonization. Based on these findings, AMF can be applied to hydroponic cultivation systems. Nevertheless, the MHB-AMF relationship should be studied further.
Areeya Mungsachat, H. A. Kalee, M. Daud et al.· Journal of Pure and Applied...· 1 citation
A controlled greenhouse study was conducted during the 2020/2021 dry season at the Cocoa Research Institute of Nigeria (CRIN), Ibadan, Nigeria, to evaluate the effects of arbuscular mycorrhizal fungi (AMF) inoculation and soil moisture on nutrient acquisition and physiological performance of three cashew (Anacardium occidentale L.) biotypes. The experiment was arranged as a 3 × 3 × 2 factorial in a completely randomized design, comprising three biotypes (Jumbo, Medium and Madras), three soil moisture levels (40%, 60% and 80% field capacity), and two AMF treatments (inoculated and non-inoculated), with three replications. AMF inoculation significantly improved foliar nutrient concentration and nutrient uptake of N, P, K, Ca and Mg, although the magnitude of response varied with biotype and moisture regime. The highest nutrient uptake and foliar nutrient concentrations generally occurred in AMF-inoculated Jumbo seedlings at 80% field capacity. AMF inoculation also improved chlorophyll content, relative water content and stomatal conductance, with the highest physiological values recorded under 80% field capacity. Jumbo generally showed the strongest response, whereas Madras recorded comparatively lower values. The findings demonstrate the potential of AMF inoculation to improve nutrient acquisition and physiological performance of cashew seedlings under varying soil moisture conditions, with the response depending on the cashew biotype and moisture regime.
O. Akanbi, I. Oyewusi, F. Asowata et al.· International Journal of Pla...· 0 citations
Overall, mycorrhizal fungal inoculation reshaped the chemical composition and the major bioactive compounds of Oregano, thereby enhancing its nutritional and antimicrobial potential.
