Morphodynamics of lipid vesicles under osmotic forcing

Abstract

We investigate the dynamics of spherical lipid vesicles subjected to an osmotic shock using continuum simulations of a permeable, area-incompressible fluid membrane with bending elasticity and surface viscosity. The dynamics are governed by a single dimensionless parameter $\varGamma$, which measures the relative strength of osmotic pressure and bending forces. Osmotic forcing drives a three-stage evolution comprising rapid buckling and collapse, smoothing of the resulting wrinkles and ridges, and slow folding into an invaginated morphology resembling the precursor to experimentally observed vesicle-in-vesicle structures. Linear stability analysis predicts the wavelength and growth rate of the initial buckling instability and shows that the dimensionless buckling time scales as $\varGamma^{-2}$, in quantitative agreement with simulations. At longer times, the energy budget reveals a reversal in elastic energy transfer: bending energy accumulated during buckling is released during relaxation and dissipated by membrane viscosity. A scaling analysis of the folding dynamics predicts that the dimensionless folding time scales as $\varGamma^{-2/3}$, again in excellent agreement with simulations. These results provide a continuum description of the mechanisms and widely separated timescales governing osmotically driven membrane collapse and invagination.