Genome-Wide Identification, Evolutionary Analysis, and Expression Profiling of the β-D-Xylosidase Gene Family in Cotton (Gossypium hirsutum) Under PEG-Simulated Osmotic and Salt Stress

Genome-wide analysis of the plant-specific PLATZ gene family in Taraxacum kok-saghyz and its roles in response to drought and salt tolerance.

It is demonstrated that heterologous expression of TksPLATZ1, TksPLATZ2 and TksPLATZ7 localize to the cell nucleus and act as transcriptional activators and repressors, respectively, which enhances the tolerance of Arabidopsis to salt and osmotic stress.

Genome-wide identification of the expansin gene family in Rosa rugosa and overexpression of RrEXPA1 contributes to drought and salt stress tolerance in Arabidopsis.

The expansin (EXs) gene family plays a crucial role in the growth and development of various plants, as well as responses to biotic and abiotic stresses. However, genome-wide analysis of the EX gene family and their functions in drought and salt stress tolerance has not been examined in Rosa rugosa. In this study, a total of 30 RrEX genes were identified and located on seven different chromosomes. Phylogenetic analysis classified these genes into four subfamilies: EXPA (24 members), EXPB (3 members), EXLA (1 member), and EXLB (2 members). The average amino acid length was 269.17 aa, with isoelectric points ranging from 4.79 to 9.97. Most members exhibited high aliphatic indices and protein stability, suggesting their adaptability to diverse environments. The synteny analysis provided insights into the evolution of the EXs gene family in rose. Toxicity and autoactivation assays confirmed that BD-RrEXPA1 was non-toxic to yeast cells and lacked autoactivation activity, indicating its suitability for yeast two-hybrid screening. The transgenic Arabidopsis lines overexpressing RrEXPA1 improved seed germination and root length under abiotic stress. In addition, the overexpression lines showed reduced malondialdehyde (MDA) levels and increased chlorophyll content and superoxide dismutase (SOD) activity. These results suggest that RrEXPA1 may enhance stress tolerance by promoting root elongation and modulating physiological responses. This study provides important insights into the role of RrEXs in salt and drought stress and lays the foundation for further studies on the regulatory mechanisms of abiotic stress.

Genome-Wide Exploration of SUPPRESSOR OF MAX2 1-LIKE Genes in Peanut (Arachis hypogaea) Reveals Developmental and Stress-responsive Expression Patterns

Genome-Wide Identification of the SWEET Gene Family in Elymus nutans and Functional Characterization of EnSWEET15 in Salt Tolerance

Overall, this study identifies EnSWEET15 as a positive regulator of salt tolerance via modulating ROS homeostasis, providing novel insights into SWEET-mediated salt stress response in perennial forages and valuable genetic resources for salt-tolerant breeding of E. nutans.

Genome-wide identi fi cation and characterization of the growth-regulating factor gene family in oat ( Avena sativa L.) and functional analysis of AsGRF17 under drought stress

Functional characterization demonstrated that transgenic tobacco plants overexpressing AsGRF17 displayed significantly enhanced drought tolerance compared with wild-type plants, and this study provides new insights into the molecular mechanisms underlying AsGRF17 -mediated drought tolerance in oat.

Genome-wide characterization of the CIPK gene family in mung bean and functional validation of VrCIPK5 in drought stress response

The heterologous overexpression of VrCIPK5 in tobacco conferred enhanced drought tolerance by promoting proline accumulation, increasing antioxidant enzyme activities, accelerating stomatal closure, and upregulating downstream stress-responsive genes, thus alleviating reactive oxygen species overaccumulation and membrane lipid peroxidation.