Tropical Journal of Natural Product Research

Abstract

level. In the present report, diosgenin quantification in tissue cultured rhizoids of D. floribunda is reported for the first time. Plantlet regeneration in vitro for vegetative propagation of some economically important Dioscorea species has been achieved using nodal cuttings 13-14 . Tissue culture is recognised as a powerful tool for plant regeneration as production is independent of season, climate or weather and one could develop true type plants, clones of the mother plant or with hormonal manipulations, modify by increasing or decreasing phytomolecule levels. Tissue culture techniques are well established in yam 15 in the quest to break yam dormancy and increase size of tubers. These studies provide a logical method for investigating the role of specific plant growth regulators (PGRs) in yam development with regards to morphogenesis and rhizogenesis. Naphthalene acetic acid (NAA) is a synthetic plant hormone in the auxin family and is a rooting agent. 6- Benzylaminopurine, benzyl adenine (BAP) is cytokinin that elicits plant growth and development responses by stimulating cell division. In plant tissue culture Indole-3-butyric acid (IBA) and other auxins are used to initiate root formation in vitro in micropropagation protocol. Kinetin is a type of cytokinin, that promotes cell division. The objective of the present