TL;DR
It is demonstrated that the maintenance of meiotic arrest in horse oocytes during culture can be promoted by theca cells, which appear to act via a secreted inhibitory factor that can not be suppressed or counteracted by FSH.
Oocyte maturation is a pivotal step in achieving successful in vitro embryo production in livestock. Among the various factors influencing in vitro maturation (IVM), the choice of protein supplement plays a crucial role in determining both the maturation rate of oocytes and their subsequent embryonic development follow...
Background: The aim of this study was to compare the effects of base medium supplemented with knock-out serum replacement (KSR; BMK group) or foetal bovine serum (FBS; BMF group) and granulosa cell conditioned medium (GCCM) prepared with KSR (CMK group) or FBS (CMF group) on in vitro maturation (IVM) of immature murine...
Pressure stimuli, such as those arising from physical forces and hydrostatic pressure in vivo, are known to regulate cellular differentiation and growth. In the ovarian microenvironment, such cues are important regulators of folliculogenesis; however, their roles during in vitro follicle growth remain unclear. This stu...
Reactive oxygen species (ROS) generated during in vitro maturation (IVM) are considered major contributors to impaired oocyte maturation and subsequent embryo development. Consequently, supplementation of IVM media with antioxidant compounds has emerged as a promising strategy for improving embryo quality. This study i...
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