Aug 2026· Discover Plants· Vol 3· 0 citations· 51 references
Abstract
Rhizobium spp. inoculation is a strategy to promote plant growth and enhance drought tolerance, particularly in semiarid environments. This study evaluated the morphological and physiological responses of Mimosa tenuiflora (Willd.) seedlings inoculated with Rhizobium spp. under different water contents. Seeds were collected from 12 naturally growing mother trees (Patos, Paraíba, Brazil). No specific genotype or cultivar was used. A completely randomized experiment was conducted in a 5 × 2 factorial design with four replicates, comprising five treatments (inoculation with three Rhizobium spp. strains: CA19PT, RO19PT, and MK17PT; nitrogen fertilization; and a non-inoculated control) under two water-content levels (40% and 80% of pot capacity). M. tenuiflora seedlings were grown in 3 dm³ pots for 90 days, after which growth, biomass production, nodulation, relative water content (RWC), and gas exchange parameters were evaluated. The results demonstrated that physiological variables were influenced by the treatments and water availability, whereas growth variables were affected only by water availability. Among the evaluated treatments, inoculation with strain CA19PT showed physiological responses comparable to those observed under nitrogen fertilization. Even under low water availability (40% pc, seedlings maintained adequate hydration levels, with an increase in root biomass. Inoculation with selected Rhizobium isolates, particularly CA19PT, improved the physiological performance of M. tenuiflora seedlings under contrasting water-content conditions. Physiological responses observed in seedlings inoculated with CA19PT were comparable to those obtained with nitrogen fertilization. Additionally, seedlings grown under higher water content (80% pc exhibit better overall growth, whereas reducing water content to 40% pcstimulates root growth.
Cowpea (Vigna unguiculata (L.) Walp.) is an agriculturally important legume capable of establishing symbiotic associations with nitrogen-fixing bacteria. Biological nitrogen fixation through Rhizobium spp. represents a potential agroecological strategy for improving crop productivity while reducing dependence on inorganic nitrogen fertilizers. The objective of this study was to evaluate the effects of Rhizobium spp. inoculation and inorganic fertilization on nodulation, plant biomass, pod production, grain yield, and 100-seed weight of cowpea under greenhouse and ex vitro conditions. Four treatments were evaluated: (1) uninoculated and unfertilized control, (2) inorganic fertilization, (3) inorganic fertilization combined with Rhizobium spp. inoculation, and (4) Rhizobium spp. inoculation without inorganic fertilizer. A total of 400 plants were evaluated throughout the experimental study. Under ex vitro conditions, plants inoculated exclusively with Rhizobium spp. produced 371 ± 104 pods, grain yield of 0.47 ± 0.14 kg m⁻², and a 100-seed weight of 25.07 ± 0.17 g. Inoculation alone also increased fresh and dry biomass by 123% and 75%, respectively, relative to the control. In contrast, combining inorganic fertilizer and Rhizobium spp. markedly reduced active nodulation relative to inoculation alone. These findings indicate that Rhizobium spp. inoculation can improve important agronomic characteristics of cowpea and represents a potential strategy for sustainable legume production and biological nitrogen management.
Unknown authors· International Journal of Sci...· 0 citations
Findings indicate that the selected halotolerant nitrogen-fixing endophytic bacterial isolates can promote rice seedling growth under saline conditions and have potential for further development as biological fertilizers for rice cultivation on saline soils.
M. Setiawati, F. Pamungkas, P. Suryatmana et al.· International Journal of Lif...· 0 citations
Panicum turgidum is an important keystone forage grass crucial for ecosystem stability in arid and semi-arid rangelands of Asia and Africa. This study aimed to investigate the use of beneficial bioinoculants for enhancing the growth and acclimatization of micropropagated desert grass under controlled environmental conditions. Tissue-cultured plants were transferred to pots with a sand–soil mixture (1:1, v/v) and kept in a temperature and light-controlled environment (25 ± 2 °C, 100 µmol m−2·s−1, and 16/8 light/dark photoperiod) for 10 weeks. We used a factorial approach to investigate the effects of the arbuscular mycorrhizal fungus (AMF) Rhizophagus fasciculatus, Trichoderma harzianum, and Bacillus subtilis on transplanted desert grass plants. Two AMF levels control (AMF, 5%; w/w) or without AMF (NAMF); and three microbial inoculation treatments (T. harzianum, B. subtilis, and a combination of T. harzianum + B. subtilis) were employed. Microscopic investigation indicated the extent of AMF colonization in the roots of micropropagated P. turgidum plantlets during acclimatization. Growth parameters with shoots and roots, chlorophyll and carotenoid content, and nitrogen uptake were all improved in bioinoculants-treated plants. Under non-AMF conditions, co-inoculation with T. harzianum and B. subtilis resulted in the highest biomass and root traits, whereas under AMF conditions, T. harzianum alone or with AMF was generally most effective. In the plants treated with the combination of inoculants, heatmap correlation, principal component analysis, and hierarchical clustering demonstrated positive association between growth and physiological traits and the absorption of mineral nutrients, although these associations varied among treatment combinations. These findings highlight the potential of beneficial bioinoculants to improve the growth and acclimatization of micropropagated desert grass plants, providing a foundation for tailored bioinoculant compositions towards the rehabilitation of degraded grazing lands.
M. M. Habib, Y. Dewir, T. Alshahrani et al.· Plants· 0 citations
Plant growth-promoting rhizobacteria consortia are used in the growth and augmentation of Mustard CS61 (Brassica juncea) under saline conditions to discover novel genes associated with stress tolerance and plant growth–promoting traits.
M. Bhat, Mayuri K Auti, Prajval Poojary· Discover Biotechnology· 0 citations
The selection of high-performing plant-bacteria partnerships, adapted to local edaphic constraints, provides a promising and eco-friendly strategy for the rehabilitation of degraded ecosystems.
Youcef Dalli, Nourredine Yahia, A. Bekki· Base· 0 citations
Reliable evaluation of rhizobial inoculant performance requires integrative assessment of nodulation and biomass responses under contrasting soil microbial environments. A controlled pot experiment was conducted to evaluate cowpea (Vigna unguiculata L. Walp.) responses to inoculation with Sinorhizobium fredii, Bradyrhizobium japonicum, mineral nitrogen (urea), and a non-inoculated control under heat-treated and unsterilized sandy loam soils. Treatments were arranged in a completely randomised design with three replicates and monitored up to 10 weeks after planting (WAP). Nodulation, vegetative growth, shoot biomass, and root biomass were assessed at 2, 4, 6, 8, and 10 WAP. Integrative indices, including nodulation biomass efficiency ratio (NBER), biomass allocation ratio (BAR), and symbiotic advantage index (SAI), were calculated at 10 WAP, and Pearson correlation analysis was performed. Nodulation differed significantly among treatments under both soil conditions (P ≤ 0.001). In heat-treated soil, S. fredii produced 45.33 nodules plant−1 compared with 27.00 nodules plant−1 under B. japonicum, while non-inoculated plants showed limited nodulation (5.00 nodules plant−1), indicating residual indigenous rhizobial influence. Under unsterilized soil, nodulation increased to 74.00 and 46.00 nodules plant−1 under S. fredii and B. japonicum, respectively. S. fredii also produced the highest shoot (5.70 g plant−1) and root biomass (2.70–2.80 g plant−1). Integrative indices significantly differentiated treatment performance, confirming S. fredii as the most effective inoculant and demonstrating the value of combined nodulation-biomass assessment for evaluating rhizobial effectiveness. Rhizobial inoculation significantly enhanced cowpea nodulation and biomass production. Sinorhizobium fredii exhibited superior nodulation and symbiotic effectiveness. Soil microbial conditions strongly influenced nodulation establishment and treatment response. Mineral nitrogen promoted vegetative growth but suppressed nodulation. Integrative indices (NBER, BAR and SAI) improved discrimination of rhizobial effectiveness. Rhizobial inoculation significantly enhanced cowpea nodulation and biomass production. Sinorhizobium fredii exhibited superior nodulation and symbiotic effectiveness. Soil microbial conditions strongly influenced nodulation establishment and treatment response. Mineral nitrogen promoted vegetative growth but suppressed nodulation. Integrative indices (NBER, BAR and SAI) improved discrimination of rhizobial effectiveness.
J. Yahaya, O. Fawole, Omoniyi Isiaq Lawal et al.· Discover Soil· 0 citations
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