LpDREB2A-LpbZIP41/LpbZIP66 module orchestrates heat stress tolerance by modulating ABA biosynthesis and carbonic anhydrase activity in perennial ryegrass.

LcNAC72‐LcDREB1 Regulatory Module Mediates Low‐Temperature Stress Response by Enhancing ROS Scavenging Capacity in Lonicera caerulea

Lonicera caerulea (blue honeysuckle) is a high‐value berry crop native to cold temperate regions with exceptional intrinsic freezing tolerance. However, research on the underlying mechanisms of cold resistance remains relatively scarce in blue honeysuckle. Here, we demonstrate that the L. caerulea transcription factors LcNAC72 interacts with LcDREB1, as revealed by yeast two‐hybrid co‐immunoprecipitation, luciferase complementation Imaging, and bimolecular fluorescence complementation assays. Agrobacterium‐mediated transient overexpression showed that LcNAC72‐LcDREB1 cooperatively modulate reactive oxygen species homeostasis to preserve cellular integrity under low temperature. Transgenic plants overexpressing LcNAC72 exhibited markedly attenuated membrane lipid peroxidation and enhanced antioxidant enzyme activity, whereas silenced plants showed exacerbated oxidative damage. Comparative transcriptomic analysis of LcNAC72‐overexpressing Arabidopsis under low temperature further demonstrated significant alteration of MAPK cascade‐related genes, suggesting a potential association of the LcNAC72‐LcDREB1 module with MAPK‐mediated signaling during cold responses. These findings provide a solid basis for exploring the genetic and molecular underpinnings of low‐temperature adaptation in L. caerulea.

From genome to gene module: decoding the BsAlfin2-BsTT2 regulatory network controlling low temperature-induced anthocyanin biosynthesis in Begonia semperflorens

This study assembled a high-quality chromosome-level B. semperflorens genome and elucidates a key molecular module in low temperature-induced anthocyanin biosynthesis regulatory pathway, laying a theoretical and data foundation for future studies on leaf color improvement and stress resistance breeding in Begonia.

Abscisic acid–regulated stability of CmABF1 and CmBRM modulates salt tolerance in chrysanthemum via epigenetic regulation of CmHSFA4

It is shown that salt stress–induced ABA accumulation up-regulates Heat Shock Factor 4 (CmHSFA4), a gene that is known to enhance chrysanthemum salt tolerance, and an ABA-SnRK2.2-ABF1/BRM signaling module that integrates phosphorylation-dependent protein stabilization and degradation with histone methylation dynamics to fine-tune salt stress–responsive gene expression in chrysanthemum is revealed.

The R2R3-MYB transcription factor ScMYB20 negatively regulates drought and salt tolerance through a dual-repression of ScCHALCONE SYNTHASE-1 (ScCHS1)-mediated flavonoid biosynthesis in the desert moss Syntrichia caninervis.

Genome-wide analysis of AP2/ERF genes identifies HpERF144 as a regulator of salt tolerance and phenylpropanoid metabolism in Herpetospermum pedunculosum.

Findings identify HpERF144 as a link between salt stress signaling and phenylpropanoid-associated metabolic regulation in a lignan-rich medicinal plant.

Genome-Wide Characterization of SnRK Gene Family in Mangrove Plant Avicennia marina and Function of AmSnRK2.7-AmENO2 Module in Response to Salinity.

Sucrose non-fermenting-1-related protein kinase (SnRK) is a plant serine/threonine kinase that mediates stress signaling, yet its function in the mangrove Avicennia marina remains unexplored. In this study, we identified 46 SnRK genes in A. marina, classifying them into three subfamilies with high phylogenetic conservation. Family expansion occurred mainly through 20 segmental duplication events under purifying selection (Ka/Ks < 1). Promoter regions were enriched in stress- and hormone-responsive elements, and expression profiling showed distinct tissue-specific and stress-responsive patterns. Our study specifically focused on the functional characterization of AmSnRK2.7. AmSnRK2.7-overexpressed Arabidopsis thaliana increases salt tolerance by altering the expression of ion transport genes and mediating Na+ efflux from the roots. Further investigation revealed that AmSnRK2.7 interacts with and phosphorylates AmENO2 (enolase, a key enzyme in the glycolysis pathway). Meanwhile, measurements of ATP content in wild type, AmSnRK2.7-overexpressed Arabidopsis lines and the atsnrk2.6 (homologous gene of AmSnRK2.7 in A. thaliana) mutant confirm that the AmSnRK2.7-AmENO2 module can affect the energy-driven Na+ efflux. Our findings provide key insights into the role of SnRK gene family in mangrove adaptation to saline intertidal habitat, and suggest AmSnRK2.7-AmENO2 module plays a role in salt tolerance.