Integrative Approaches in Protein Structure/Function Analysis, Design, and Engineering.

Protein Engineering: Principles, Strategies and Applications in Biotechnology

Protein engineering is a rapidly developing field of biotechnology that involves the modification or redesign of proteins to obtain improved, altered, or novel properties. The ability to engineer proteins has significantly expanded their applications in industrial biotechnology, medicine, agriculture, food technology,...

Machine Learning Empowers Enzyme Discovery, Structure Prediction, Engineering, and Design

Enzymes are efficient and selective biocatalysts, yet obtaining enzymes with desired properties remains challenging due to vast sequence spaces, incomplete functional annotation, limited structural information, and costly experimental screening. Machine learning (ML) offers a powerful complement to address these limi...

Promoter Engineering in Yeast Cell Factories: Strategies, Applications, and Perspectives

Yeasts serve as premier eukaryotic hosts for microbial cell factories, enabling the production of recombinant proteins, biofuels, and high-value natural products. Precise transcriptional control is paramount for balancing complex metabolic pathways and maximizing target product yields. Promoters, as the key regulator...

Synthetic Biology Tools and Strategies for Microbial Cell Factory Construction.

Designing a DeepPCD Strategy for Efficient Engineering Nitrilases toward Industrial Bioconversion

The integration of artificial intelligence with protein engineering has created unprecedented opportunities for enzyme discovery and the enhancement of overall catalytic performance. In this study, a novel nitrilase, BhNIT, was identified from Bradyrhizobiumhuanghuaihaiense through a computationally assisted screenin...

Advances in directed evolution: Mutagenesis, sensing, screening, and in silico innovation.

Directed evolution (DE) represents a pivotal interdisciplinary paradigm that synergistically integrates biotechnology, computational methodologies, and precision analytical instrumentation. It enables accelerated, controllable in vitro laboratory evolution of diverse biomolecules toward predefined functional targets. T...