Open access
A Bioconjugable Eu(III)-Based Luminescent Probe for Multicolor and Time-Gated Immunofluorescence
Francesco Busato
Martina De Felice
Filippo Petri
Mattia Fausti
Blanca Bruschi
Pietro Lenzi
Francesca Cella Zanacchi
D. Pisignano
L. di Bari
L. Arrico
Abstract
We present a π-conjugated ligand endowed with blue emission and the ability to form a self-assembled red-emitting europium(III) complex with a long emission lifetime (430 μs), even in a complex cellular environment. The ligand is easily synthesized, readily conjugated to antibodies, and suitable for multicolor and time-gated fluorescence microscopy, offering the possibility of imaging cellular structures in distinct spectral and temporal windows.