Resource Announcement of Five Rhizoctonia solani Genomes Isolated from Tobacco

Abstract

Rhizoctonia solani is a filamentous fungal pathogen that causes damping-off, sore shin, and target spot in tobacco. Limited genomic resources for tobacco-associated isolates from the United States (U.S.) have hindered studies of evolutionary relationships, host adaptation, and pathogenicity. Here, we report whole-genome assemblies of R. solani isolates representing anastomosis groups (AGs) 3 and 4 isolated from tobacco in Virginia and North Carolina, two leading flue-cured tobacco-producing states in the U.S. AG‑3 assemblies ranged from 39.05 to 39.46 Mb with 11,400 to 11,505 predicted genes and BUSCO completeness of 94.9% to 95.2%. The AG‑4 genome was larger at 44.70 Mb with 12,831 genes and 95.4% BUSCO completeness. These five genomes expand the publicly available set of U.S. tobacco-associated R. solani, including the first tobacco-derived AG-4 genome, and provide a foundation for future studies of host adaptation and virulence variations in this species complex.