TL;DR
This chapter discusses label-free optical imaging applications with AI-assisted approaches, specifically in cancer diagnosis, cell line selection for biopharmaceuticals, and clinical ear infection diagnosis using SLAM, multimodal nonlinear imaging (SLAM and FLIM), and OCT, respectively.
Nonlinear imaging such as third-harmonic generation (THG) microscopy can produce label-free images of tissue with histopathological quality, yet the interpretation of label-free images is challenging. Virtual staining can convert label-free images into histology-like images, but its application to nonlinear imaging rem...
Multimodal fluorescence and phase imaging provides complementary information about biological samples. However, existing simultaneous fluorescence-phase techniques primarily operate in transmission mode and are limited to thin specimens, restricting their use in thick tissues and in vivo applications. Although mult...
Label-free interferometric imaging avoids labelling agents and instead exploits intrinsic optical properties of biological samples, making it a general and unbiased alternative to label-based imaging, particularly when suitable exogenous markers are unknown or unavailable. By providing quantitative phase and refractive...
In vivo fluorescence lifetime imaging (FLI) is an advanced optical imaging modality with the capability to reveal additional biological information compared to fluorescence intensity (FI) imaging. In addition to enabling tissue discrimination based on microenvironmental variations, lifetime contrast allows the differen...
ABSTRACT Two‐photon microscopy (TPM) has revolutionized biological imaging by enabling high‐resolution, deep‐tissue visualization with reduced photobleaching and photodamage. This review discusses complementary nonlinear optical imaging modalities such as two‐photon fluorescence (TPF), second‐harmonic generation (SHG),...
Semiconductor metrology and inspection increasingly require characterization techniques capable of resolving nanoscale structures while simultaneously providing chemical and functional information under realistic operating conditions. Although electron and scanning probe microscopy techniques offer outstanding spatial...
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